This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Assume that you grew a culture of E. coli for many generations in medium containing 15N (from the ... (Read 102 times)

jc611

  • Hero Member
  • *****
  • Posts: 552
on: Mar 26, 2020
Assume that you grew a culture of E. coli for many generations in medium containing 15N (from the ammonium ion), a heavy isotope of nitrogen. You extract DNA from a portion of the culture and determine its density to be 1.723 gm/cm3 (call this sample A). You then wash the remaining E. coli cells and grow them for one generation in 14N, extract the DNA from a portion of the culture, and determine its density to be 1.715 gm/cm3 (call this sample B). You let the culture grow for one more generation in 14N and extract the DNA (call this sample C). Each sample of DNA (A, B, and C) is then heated to completely denature the double-stranded structures, cooled quickly (to keep the strands separate), and subjected to ultracentrifugation. Present the centrifugation profiles for heat-denatured DNA (samples A, B, and C) that you would expect. Use the graph below. (Note: Although not the case, assume that single-stranded DNA has the same density as double-stranded DNA.)

Essentials of Genetics ¦ Klug ¦ 9th Edition
Buy Test-Bank


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by jc611 on Mar 26, 2020

Chelseyj.hasty

  • Sr. Member
  • ****
  • Posts: 319
Reply #1 on: Mar 26, 2020
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
Answer Preview
Only 25% of students answer this correctly


jc611

  • Member
  • Posts: 552
Reply 2 on: Mar 26, 2020
Wow, this really help

Chelseyj.hasty

  • Member
  • Posts: 319
Reply 3 on: Yesterday
Great answer, keep it coming :)

« previous next »
 

Did you know?

An identified risk factor for osteoporosis is the intake of excessive amounts of vitamin A. Dietary intake of approximately double the recommended daily amount of vitamin A, by women, has been shown to reduce bone mineral density and increase the chances for hip fractures compared with women who consumed the recommended daily amount (or less) of vitamin A.

Did you know?

Anti-aging claims should not ever be believed. There is no supplement, medication, or any other substance that has been proven to slow or stop the aging process.

Did you know?

Most childhood vaccines are 90–99% effective in preventing disease. Side effects are rarely serious.

Did you know?

The most common childhood diseases include croup, chickenpox, ear infections, flu, pneumonia, ringworm, respiratory syncytial virus, scabies, head lice, and asthma.

Did you know?

Never take aspirin without food because it is likely to irritate your stomach. Never give aspirin to children under age 12. Overdoses of aspirin have the potential to cause deafness.

Single E. coli bacterium Single E. coli bacterium
Triumphal Entry, page from a manuscript of Firdawsi's Shahnamah, Persian, Safavid culture. Triumphal Entry, page from a manuscript of Firdawsi's Shahnamah, Persian, Safavid culture.
Role-Playing a Situation in Which a Patient Is from Another Culture Role-Playing a Situation in Which a Patient Is from Another Culture
Except for interaction within families, age groups in Western culture are usually kept fairly ... Except for interaction within families, age groups in Western culture are usually kept fairly ...
Religion often helps immigrants adapt to their new culture. What indications of this do you see in ... Religion often helps immigrants adapt to their new culture. What indications of this do you see in ...
The cell wall of a Gram-negative bacterium, E. coli The cell wall of a Gram-negative bacterium, E. coli
Video: Mycobacterium tuberculosis: Drug Resistance and Natural Selection

For a complete list of videos, visit our video library