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Author Question: Assume that you grew a culture of E. coli for many generations in medium containing 15N (from the ... (Read 40 times)

jc611

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on: Mar 26, 2020
Assume that you grew a culture of E. coli for many generations in medium containing 15N (from the ammonium ion), a heavy isotope of nitrogen. You extract DNA from a portion of the culture and determine its density to be 1.723 gm/cm3 (call this sample A). You then wash the remaining E. coli cells and grow them for one generation in 14N, extract the DNA from a portion of the culture, and determine its density to be 1.715 gm/cm3 (call this sample B). You let the culture grow for one more generation in 14N and extract the DNA (call this sample C). Each sample of DNA (A, B, and C) is then heated to completely denature the double-stranded structures, cooled quickly (to keep the strands separate), and subjected to ultracentrifugation. Present the centrifugation profiles for heat-denatured DNA (samples A, B, and C) that you would expect. Use the graph below. (Note: Although not the case, assume that single-stranded DNA has the same density as double-stranded DNA.)

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Marked as best answer by jc611 on Mar 26, 2020

Chelseyj.hasty

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Reply #1 on: Mar 26, 2020
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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jc611

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Reply 2 on: Mar 26, 2020
Thanks for the timely response, appreciate it

kusterl

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Reply 3 on: Yesterday
Wow, this really help

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