Question 1
What primary ingredients, coupled with DNA polymerase I, are needed for the
in vitro synthesis of DNA?
Question 2
Refer to the accompanying diagram of a generalized tetranucleotide to answer questions (a) through (e).
(a) Is this a DNA or an RNA molecule?
(b) Place an "X" (in one of the circles in the diagram) at the 3′ end of this tetranucleotide.
(c) Given that the DNA strand, which served as a template for the synthesis of this tetranucleotide, was composed of the bases 5′ — A C A G — 3′, fill in the parentheses (in the diagram) with the expected bases.
(d) Suppose that one of the precursors for this tetranucleotide was a
32P-labeled guanine nucleoside triphosphate (the innermost phosphate containing the radioactive phosphorus). Circle the radioactive phosphorus atom as it exists in the tetranucleotide.
(e) Given that spleen diesterase (breaks between the phosphate and the 5′ carbon) digests the pictured tetranucleotide, which base(s) among the breakdown products would be expected to be attached to the
32P?
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