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Author Question: If bacteria transformed with a recombinant pUC18 plasmid produce white colonies when grown on plates ... (Read 49 times)

vicky

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on: Jan 12, 2022

Question 1

Half of the palindromic restriction site that the endonuclease PstI recognizes, binds, and cleaves is 5'-CTG________-3'. What is the complete sequence?
◦ 5'-CTGCTG-3'
◦ 5'-CTGGTC-3'
◦ 5'-CTGCAG-3'
◦ 5'-CTGGAG-3'
◦ 5'-CTGGAC-3'

Question 2

If bacteria transformed with a recombinant pUC18 plasmid produce white colonies when grown on plates containing ampicillin and Xgal, which of the following is least likely?
◦ They produce β-lactamase that provides resistance to ampicillin.
◦ They produce functional β-galactosidase that cleaves X-gal.
◦ They carry a vector with the bla gene used as the selectable marker.
◦ They carry a vector that contains a DNA fragment inserted into the multiple cloning site.
◦ They carry a vector with a lacZ gene that has been disrupted and rendered nonfunctional.
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Marked as best answer by vicky on Jan 12, 2022

AngeliqueG

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Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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vicky

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Reply 2 on: Jan 12, 2022
Excellent

tranoy

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Reply 3 on: Yesterday
Thanks for the timely response, appreciate it

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