Question 1
Half of the palindromic restriction site that the endonuclease
PstI recognizes, binds, and cleaves is 5'-CTG________-3'. What is the complete sequence?
◦ 5'-CTGCTG-3'
◦ 5'-CTGGTC-3'
◦ 5'-CTGCAG-3'
◦ 5'-CTGGAG-3'
◦ 5'-CTGGAC-3'
Question 2
If bacteria transformed with a recombinant pUC18 plasmid produce white colonies when grown on plates containing ampicillin and Xgal, which of the following is least likely?
◦ They produce β-lactamase that provides resistance to ampicillin.
◦ They produce functional β-galactosidase that cleaves X-gal.
◦ They carry a vector with the
bla gene used as the selectable marker.
◦ They carry a vector that contains a DNA fragment inserted into the multiple cloning site.
◦ They carry a vector with a
lacZ gene that has been disrupted and rendered nonfunctional.
Using the Ti plasmid and Agrobacterium tumefaciens to make transgenic plants
White matter
White footed mouse (Peromyscus leucopus)
Results of the serial dilution technique and subsequent culture of bacteria