Question 1
Half of the palindromic restriction site that the endonuclease
PstI recognizes, binds, and cleaves is 5'-CTG________-3'. What is the complete sequence?
◦ 5'-CTGCTG-3'
◦ 5'-CTGGTC-3'
◦ 5'-CTGCAG-3'
◦ 5'-CTGGAG-3'
◦ 5'-CTGGAC-3'
Question 2
If bacteria transformed with a recombinant pUC18 plasmid produce white colonies when grown on plates containing ampicillin and Xgal, which of the following is least likely?
◦ They produce β-lactamase that provides resistance to ampicillin.
◦ They produce functional β-galactosidase that cleaves X-gal.
◦ They carry a vector with the
bla gene used as the selectable marker.
◦ They carry a vector that contains a DNA fragment inserted into the multiple cloning site.
◦ They carry a vector with a
lacZ gene that has been disrupted and rendered nonfunctional.
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