This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Which of the following best lists the correct order of the steps taken to produce human insulin ... (Read 97 times)

jho37

  • Hero Member
  • *****
  • Posts: 531
on: Jan 12, 2022
Which of the following best lists the correct order of the steps taken to produce human insulin in E.coli in the 1970s?

I.Each insulin chain was cloned into an expression vector as a continuation of the lacZ reading 
frame, resulting in a fusion protein for each chain.
II.Disulfide bonds were induced to form between the cysteine residues of the purified insulin A 
and B chains.
III.Both human insulin genes were reverse translated to create synthetic genes.
IV.EcoRI and BamHI restriction sites were added to the ends of the DNA to facilitate directional 
cloning of each into a separate plasmid vector.

◦ I, II, III, IV
◦ III, IV, I, II
◦ IV, III, I, II
◦ III, I, II, IV
◦ I, III, IV, II
Genetic Analysis: An Integrated Approach ¦ Sanders, Bowman ¦ 3rd Edition
Buy Test-Bank


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by jho37 on Jan 12, 2022

ririgirl15

  • Sr. Member
  • ****
  • Posts: 385
Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
Answer Preview
Only 39% of students answer this correctly


jho37

  • Member
  • Posts: 531
Reply 2 on: Jan 12, 2022
Thanks for the timely response, appreciate it

nathang24

  • Member
  • Posts: 314
Reply 3 on: Yesterday
:D TYSM

« previous next »
 

Did you know?

ACTH levels are normally highest in the early morning (between 6 and 8 A.M.) and lowest in the evening (between 6 and 11 P.M.). Therefore, a doctor who suspects abnormal levels looks for low ACTH in the morning and high ACTH in the evening.

Did you know?

Symptoms of kidney problems include a loss of appetite, back pain (which may be sudden and intense), chills, abdominal pain, fluid retention, nausea, the urge to urinate, vomiting, and fever.

Did you know?

Pregnant women usually experience a heightened sense of smell beginning late in the first trimester. Some experts call this the body's way of protecting a pregnant woman from foods that are unsafe for the fetus.

Did you know?

The strongest synthetic topical retinoid drug available, tazarotene, is used to treat sun-damaged skin, acne, and psoriasis.

Did you know?

Multiple experimental evidences have confirmed that at the molecular level, cancer is caused by lesions in cellular DNA.

A comparison of the proteomes in human liver and skeletal muscle cells A comparison of the proteomes in human liver and skeletal muscle cells
Human Heart Model Human Heart Model
Human hemoglobin variants Human hemoglobin variants
Steps in acute inflammation Steps in acute inflammation
Steps in protein synthesis: The DNA is used as a template to make mRNA (transcription). The mRNA is ... Steps in protein synthesis: The DNA is used as a template to make mRNA (transcription). The mRNA is ...
Directions: Fill in the correct number or word(s). Order: Lantus U-100 insulin 68 units subcut ... Directions: Fill in the correct number or word(s). Order: Lantus U-100 insulin 68 units subcut ...
Video: Oxygen - hemoglobin dissociation curve & Hemoglobin's affinity with oxygen - acidity

For a complete list of videos, visit our video library