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Author Question: Which of the following lists the steps for cDNA library preparation in the correct order?I.Partially ... (Read 111 times)

bb

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on: Jan 12, 2022
Which of the following lists the steps for cDNA library preparation in the correct order?

I.Partially degrade the mRNA with RNase H
II.Isolate mRNA from a specific cell/ tissue with an oligo-dT primer that hybridizes to poly-A tail
III.Synthesize a strand of cDNA using DNA polymerase with mRNA fragments as primers
IV.Ligate cDNAs into a cloning vector
V.Create a single-stranded DNA molecule complementary to the mRNA using reverse 
transcriptase

◦ I, II, III, IV, V
◦ II, V, I, III, IV
◦ II, III, V, I, IV
◦ V, III, I, IV, II
◦ II, V, IV, III, I
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Marked as best answer by bb on Jan 12, 2022

vickybb89

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Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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bb

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Reply 2 on: Jan 12, 2022
Thanks for the timely response, appreciate it

matt95

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Reply 3 on: Yesterday
Excellent

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