This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Which of the following lists the steps for cDNA library preparation in the correct order?I.Partially ... (Read 53 times)

bb

  • Hero Member
  • *****
  • Posts: 544
on: Jan 12, 2022
Which of the following lists the steps for cDNA library preparation in the correct order?

I.Partially degrade the mRNA with RNase H
II.Isolate mRNA from a specific cell/ tissue with an oligo-dT primer that hybridizes to poly-A tail
III.Synthesize a strand of cDNA using DNA polymerase with mRNA fragments as primers
IV.Ligate cDNAs into a cloning vector
V.Create a single-stranded DNA molecule complementary to the mRNA using reverse 
transcriptase

◦ I, II, III, IV, V
◦ II, V, I, III, IV
◦ II, III, V, I, IV
◦ V, III, I, IV, II
◦ II, V, IV, III, I
Genetic Analysis: An Integrated Approach ¦ Sanders, Bowman ¦ 3rd Edition
Buy Test-Bank


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by bb on Jan 12, 2022

vickybb89

  • Sr. Member
  • ****
  • Posts: 347
Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
Answer Preview
Only 39% of students answer this correctly


bb

  • Member
  • Posts: 544
Reply 2 on: Jan 12, 2022
Wow, this really help

phuda

  • Member
  • Posts: 348
Reply 3 on: Yesterday
Thanks for the timely response, appreciate it

« previous next »
 

Did you know?

If you use artificial sweeteners, such as cyclamates, your eyes may be more sensitive to light. Other factors that will make your eyes more sensitive to light include use of antibiotics, oral contraceptives, hypertension medications, diuretics, and antidiabetic medications.

Did you know?

The Centers for Disease Control and Prevention has released reports detailing the deaths of infants (younger than 1 year of age) who died after being given cold and cough medications. This underscores the importance of educating parents that children younger than 2 years of age should never be given over-the-counter cold and cough medications without consulting their physicians.

Did you know?

Only one in 10 cancer deaths is caused by the primary tumor. The vast majority of cancer mortality is caused by cells breaking away from the main tumor and metastasizing to other parts of the body, such as the brain, bones, or liver.

Did you know?

Medications that are definitely not safe to take when breastfeeding include radioactive drugs, antimetabolites, some cancer (chemotherapy) agents, bromocriptine, ergotamine, methotrexate, and cyclosporine.

Did you know?

On average, the stomach produces 2 L of hydrochloric acid per day.

British Library British Library
Creede, Colorado, circa 1890, a rough silver mining town that grew from 660 people in 1889 to over 1 Creede, Colorado, circa 1890, a rough silver mining town that grew from 660 people in 1889 to over 1
If the valve clearance (lash) is not correct, loosen the retaining nut and turn the valve adjusting ... If the valve clearance (lash) is not correct, loosen the retaining nut and turn the valve adjusting ...
Growth cones. The cytoplasmic fingers (the filopodia) of growth cones seem to grope for the correct ... Growth cones. The cytoplasmic fingers (the filopodia) of growth cones seem to grope for the correct ...
Preparation and resealing of erythrocyte ghosts Preparation and resealing of erythrocyte ghosts
Initiation and elongation steps of transcription by bacterial RNA polymerase Initiation and elongation steps of transcription by bacterial RNA polymerase
Video: Predator- prey competition and symbiosis

For a complete list of videos, visit our video library