Author Question: What principle does the Sysmex SE-9000/9500 use to analyze and separate white cell populations? ... (Read 54 times)

Cooldude101 · **on:** Jun 25, 2018

What principle does the Sysmex SE-9000/9500 use to analyze and separate white cell populations?

a. Electronic impedance
b. Optical light scatter
c. Volume, conductivity, and scatter
d. Low-frequency direct current and high-frequency current

Question 2

What technology minimizes the problems of a rigid aperture including protein buildup, coincidence, and a single cell being counted twice?

a. Forward scatter
b. Radiofrequency
c. Hydrodynamic focusing
d. Fluorescence

dreamfighter72 · **Reply #1 on:** Jun 25, 2018

Answer to Question 1

ANS: D
The Sysmex 9000 analyzes WBCs by low-frequency direct current and high-frequency current. A scattergram of radiofrequency detection signals (y-axis) versus direct-current detection signals (x-axis) is plotted and yields separation of the white cells through the application of floating discriminators.

Answer to Question 2

ANS: C
The use of hydrodynamic focusing avoids the pitfalls and potential problems when using a rigid aperture. The outer sheath fluid minimizes protein buildup and plugs, eliminates recirculation of cells, and reduces pulse height irregularity and coincident passage loss.