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Author Question: What principle does the Sysmex SE-9000/9500 use to analyze and separate white cell populations? ... (Read 22 times)

Cooldude101

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on: Jun 25, 2018
What principle does the Sysmex SE-9000/9500 use to analyze and separate white cell populations?
 
  a. Electronic impedance
  b. Optical light scatter
  c. Volume, conductivity, and scatter
  d. Low-frequency direct current and high-frequency current

Question 2

What technology minimizes the problems of a rigid aperture including protein buildup, coincidence, and a single cell being counted twice?
 
  a. Forward scatter
  b. Radiofrequency
  c. Hydrodynamic focusing
  d. Fluorescence


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dreamfighter72

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Reply #1 on: Jun 25, 2018
Answer to Question 1

ANS: D
The Sysmex 9000 analyzes WBCs by low-frequency direct current and high-frequency current. A scattergram of radiofrequency detection signals (y-axis) versus direct-current detection signals (x-axis) is plotted and yields separation of the white cells through the application of floating discriminators.

Answer to Question 2

ANS: C
The use of hydrodynamic focusing avoids the pitfalls and potential problems when using a rigid aperture. The outer sheath fluid minimizes protein buildup and plugs, eliminates recirculation of cells, and reduces pulse height irregularity and coincident passage loss.


Cooldude101

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Reply 2 on: Jun 25, 2018
Thanks for the timely response, appreciate it

scottmt

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Reply 3 on: Yesterday
Great answer, keep it coming :)

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