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Author Question: What is the preferred specimen for molecular techniques for diagnosis of inherited mutations? a. ... (Read 105 times)

ishan

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on: Jun 25, 2018
What is the preferred specimen for molecular techniques for diagnosis of inherited mutations?
 
  a. RNA extracted from peripheral mature red cells
  b. RNA extracted from fresh serum
  c. DNA extracted from peripheral blood white cells
  d. DNA extracted from peripheral blood mature red cells

Question 2

How does ribonucleic acid (RNA) differ from deoxyribonucleic acid (DNA)?
 
  a. RNA does not contain nucleotides.
  b. RNA has uracil-replacing thymine.
  c. DNA is found primarily in the cytoplasm, and RNA is in the nucleus.
  d. DNA has a messenger, whereas RNA does not.


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kthug

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Reply #1 on: Jun 25, 2018
Answer to Question 1

ANS: C
DNA extracted from peripheral blood (or bone marrow) white cells is the preferred specimen for molecular techniques when they are used to diagnose inherited disorders. In inherited disorders, the DNA from any cell could theoretically be used because the DNA at the molecular level will be identical regardless of cell origin. However, DNA from peripheral blood white cells is the easiest tissue to obtain. Mature red blood cells (RBCs) cannot be used because they do not have a nucleus and therefore do not have any DNA.

Answer to Question 2

ANS: B
RNA differs from DNA in that it has uracil-replacing thymine.


ishan

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Reply 2 on: Jun 25, 2018
Thanks for the timely response, appreciate it

bigsis44

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Reply 3 on: Yesterday
Great answer, keep it coming :)

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