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Author Question: Explain how the activity of brain cells is measured using autoradiography. What will be an ideal ... (Read 116 times)

Starlight

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on: Jun 22, 2018
Explain how the activity of brain cells is measured using autoradiography.
 
  What will be an ideal response?

Question 2

Explain the rationale behind the optogenetic methods.
 
  What will be an ideal response?


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cici

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Reply #1 on: Jun 22, 2018
Answer to Question 1

Answer: Brain cells require glucose as fuel for activity. 2-DG, like glucose, is taken up into neurons, but cannot be used for energy. If a radioactive version of 2-DG is injected into an animal, radioactive 2-DG molecules will accumulate in the brain as a function of the relative activity of those cells. Autoradiography will reveal the presence of radioactive molecules in the brain, with a more intense signal in regions that were more active and a less intense signal in less active regions.

Answer to Question 2

Answer: The method can be used to stimulate or inhibit neurons in a specific brain region. Two distinct photosensitive proteins have been identified. ChR2 controls a sodium channel and is sensitive to blue light. NpHR controls a chloride channel and is sensitive to yellow light. The proteins are inserted into a brain region and the response to blue or yellow light is noted. Typically, blue light activates and yellow light inhibits neuron activity.


Starlight

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Reply 2 on: Jun 22, 2018
Gracias!

scikid

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Reply 3 on: Yesterday
:D TYSM

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