This topic contains a solution. Click here to go to the answer

Author Question: Explain how the activity of brain cells is measured using autoradiography. What will be an ideal ... (Read 102 times)

Starlight

  • Hero Member
  • *****
  • Posts: 567
Explain how the activity of brain cells is measured using autoradiography.
 
  What will be an ideal response?

Question 2

Explain the rationale behind the optogenetic methods.
 
  What will be an ideal response?



Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by a Subject Expert

cici

  • Sr. Member
  • ****
  • Posts: 325
Answer to Question 1

Answer: Brain cells require glucose as fuel for activity. 2-DG, like glucose, is taken up into neurons, but cannot be used for energy. If a radioactive version of 2-DG is injected into an animal, radioactive 2-DG molecules will accumulate in the brain as a function of the relative activity of those cells. Autoradiography will reveal the presence of radioactive molecules in the brain, with a more intense signal in regions that were more active and a less intense signal in less active regions.

Answer to Question 2

Answer: The method can be used to stimulate or inhibit neurons in a specific brain region. Two distinct photosensitive proteins have been identified. ChR2 controls a sodium channel and is sensitive to blue light. NpHR controls a chloride channel and is sensitive to yellow light. The proteins are inserted into a brain region and the response to blue or yellow light is noted. Typically, blue light activates and yellow light inhibits neuron activity.




Starlight

  • Member
  • Posts: 567
Reply 2 on: Jun 22, 2018
Great answer, keep it coming :)


xiazhe

  • Member
  • Posts: 331
Reply 3 on: Yesterday
Gracias!

 

Did you know?

Hypertension is a silent killer because it is deadly and has no significant early symptoms. The danger from hypertension is the extra load on the heart, which can lead to hypertensive heart disease and kidney damage. This occurs without any major symptoms until the high blood pressure becomes extreme. Regular blood pressure checks are an important method of catching hypertension before it can kill you.

Did you know?

Between 1999 and 2012, American adults with high total cholesterol decreased from 18.3% to 12.9%

Did you know?

The average human gut is home to perhaps 500 to 1,000 different species of bacteria.

Did you know?

About 3.2 billion people, nearly half the world population, are at risk for malaria. In 2015, there are about 214 million malaria cases and an estimated 438,000 malaria deaths.

Did you know?

Computer programs are available that crosscheck a new drug's possible trade name with all other trade names currently available. These programs detect dangerous similarities between names and alert the manufacturer of the drug.

For a complete list of videos, visit our video library