This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Explain how the activity of brain cells is measured using autoradiography. What will be an ideal ... (Read 46 times)

Starlight

  • Hero Member
  • *****
  • Posts: 567
on: Jun 22, 2018
Explain how the activity of brain cells is measured using autoradiography.
 
  What will be an ideal response?

Question 2

Explain the rationale behind the optogenetic methods.
 
  What will be an ideal response?


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by a Subject Expert

cici

  • Sr. Member
  • ****
  • Posts: 325
Reply #1 on: Jun 22, 2018
Answer to Question 1

Answer: Brain cells require glucose as fuel for activity. 2-DG, like glucose, is taken up into neurons, but cannot be used for energy. If a radioactive version of 2-DG is injected into an animal, radioactive 2-DG molecules will accumulate in the brain as a function of the relative activity of those cells. Autoradiography will reveal the presence of radioactive molecules in the brain, with a more intense signal in regions that were more active and a less intense signal in less active regions.

Answer to Question 2

Answer: The method can be used to stimulate or inhibit neurons in a specific brain region. Two distinct photosensitive proteins have been identified. ChR2 controls a sodium channel and is sensitive to blue light. NpHR controls a chloride channel and is sensitive to yellow light. The proteins are inserted into a brain region and the response to blue or yellow light is noted. Typically, blue light activates and yellow light inhibits neuron activity.


Starlight

  • Member
  • Posts: 567
Reply 2 on: Jun 22, 2018
Excellent

31809pancho

  • Member
  • Posts: 317
Reply 3 on: Yesterday
Great answer, keep it coming :)

« previous next »
 

Did you know?

A serious new warning has been established for pregnant women against taking ACE inhibitors during pregnancy. In the study, the risk of major birth defects in children whose mothers took ACE inhibitors during the first trimester was nearly three times higher than in children whose mothers didn't take ACE inhibitors. Physicians can prescribe alternative medications for pregnant women who have symptoms of high blood pressure.

Did you know?

The B-complex vitamins and vitamin C are not stored in the body and must be replaced each day.

Did you know?

There used to be a metric calendar, as well as metric clocks. The metric calendar, or "French Republican Calendar" divided the year into 12 months, but each month was divided into three 10-day weeks. Each day had 10 decimal hours. Each hour had 100 decimal minutes. Due to lack of popularity, the metric clocks and calendars were ended in 1795, three years after they had been first marketed.

Did you know?

A cataract is a clouding of the eyes' natural lens. As we age, some clouding of the lens may occur. The first sign of a cataract is usually blurry vision. Although glasses and other visual aids may at first help a person with cataracts, surgery may become inevitable. Cataract surgery is very successful in restoring vision, and it is the most frequently performed surgery in the United States.

Did you know?

The tallest man ever known was Robert Wadlow, an American, who reached the height of 8 feet 11 inches. He died at age 26 years from an infection caused by the immense weight of his body (491 pounds) and the stress on his leg bones and muscles.

blood cells in the artery blood cells in the artery
SHEEP BRAIN SHEEP BRAIN
Brain Dissection Brain Dissection
Adenosine triphosphate (ATP) is the most common molecule used by cells to captur Adenosine triphosphate (ATP) is the most common molecule used by cells to captur
The bridging ability of IgM: (a) Pictorial view of IgM bridging of red cells. (b) Macroscopic view ... The bridging ability of IgM: (a) Pictorial view of IgM bridging of red cells. (b) Macroscopic view ...
Explain how it can be desirable from an intertemporal perspective for a country run a current ... Explain how it can be desirable from an intertemporal perspective for a country run a current ...
Video: T cell receptors

For a complete list of videos, visit our video library