This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Explain how the activity of brain cells is measured using autoradiography. What will be an ideal ... (Read 103 times)

Starlight

  • Hero Member
  • *****
  • Posts: 567
on: Jun 22, 2018
Explain how the activity of brain cells is measured using autoradiography.
 
  What will be an ideal response?

Question 2

Explain the rationale behind the optogenetic methods.
 
  What will be an ideal response?


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by a Subject Expert

cici

  • Sr. Member
  • ****
  • Posts: 325
Reply #1 on: Jun 22, 2018
Answer to Question 1

Answer: Brain cells require glucose as fuel for activity. 2-DG, like glucose, is taken up into neurons, but cannot be used for energy. If a radioactive version of 2-DG is injected into an animal, radioactive 2-DG molecules will accumulate in the brain as a function of the relative activity of those cells. Autoradiography will reveal the presence of radioactive molecules in the brain, with a more intense signal in regions that were more active and a less intense signal in less active regions.

Answer to Question 2

Answer: The method can be used to stimulate or inhibit neurons in a specific brain region. Two distinct photosensitive proteins have been identified. ChR2 controls a sodium channel and is sensitive to blue light. NpHR controls a chloride channel and is sensitive to yellow light. The proteins are inserted into a brain region and the response to blue or yellow light is noted. Typically, blue light activates and yellow light inhibits neuron activity.


Starlight

  • Member
  • Posts: 567
Reply 2 on: Jun 22, 2018
Great answer, keep it coming :)

mcabuhat

  • Member
  • Posts: 344
Reply 3 on: Yesterday
YES! Correct, THANKS for helping me on my review

« previous next »
 

Did you know?

When intravenous medications are involved in adverse drug events, their harmful effects may occur more rapidly, and be more severe than errors with oral medications. This is due to the direct administration into the bloodstream.

Did you know?

Once thought to have neurofibromatosis, Joseph Merrick (also known as "the elephant man") is now, in retrospect, thought by clinical experts to have had Proteus syndrome. This endocrine disease causes continued and abnormal growth of the bones, muscles, skin, and so on and can become completely debilitating with severe deformities occurring anywhere on the body.

Did you know?

Asthma attacks and symptoms usually get started by specific triggers (such as viruses, allergies, gases, and air particles). You should talk to your doctor about these triggers and find ways to avoid or get rid of them.

Did you know?

Despite claims by manufacturers, the supplement known as Ginkgo biloba was shown in a study of more than 3,000 participants to be ineffective in reducing development of dementia and Alzheimer’s disease in older people.

Did you know?

There are more nerve cells in one human brain than there are stars in the Milky Way.

Communication by direct contact between cells. Communication by direct contact between cells.
Brain, spinal cord, and spinal nerves with an expanded view of a spinal nerve. Brain, spinal cord, and spinal nerves with an expanded view of a spinal nerve.
THE BRAIN THE BRAIN
Grading of Cancer Cells Grading of Cancer Cells
ATP, an important energy currency in cells ATP, an important energy currency in cells
Video: Primate Evolutionary Tree Animation

For a complete list of videos, visit our video library