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Author Question: Why are leading and lagging strand primers removed rather than joined with Okazaki fragments? (Read 84 times)

HudsonKB16

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on: Jan 12, 2022

Question 1

Which of the following would you find in a Sanger sequencing reaction but not in a polymerase chain reaction?
◦ DNA template
◦ DNA polymerase
◦ dNTPs
◦ ddNTPs
◦ DNA primer

Question 2

Why are leading and lagging strand primers removed rather than joined with Okazaki fragments?
◦ The primers do not hydrogen bond to the template strand correctly.
◦ They have to be removed for leading and lagging strand synthesis to begin.
◦ Primers are too short to efficiently bind to Okazaki fragments.
◦ They contain nucleotides with 2'OH groups, and are targeted for excision by DNA Polymerase.
◦ They lack the 3' OH that would permit them to be covalently linked to the Okazaki fragments.
Genetic Analysis: An Integrated Approach ¦ Sanders, Bowman ¦ 3rd Edition
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Marked as best answer by HudsonKB16 on Jan 12, 2022

sabina576

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Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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HudsonKB16

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Reply 2 on: Jan 12, 2022
Great answer, keep it coming :)

scikid

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Reply 3 on: Yesterday
Wow, this really help

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