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Author Question: Why are leading and lagging strand primers removed rather than joined with Okazaki fragments? (Read 23 times)

HudsonKB16

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on: Jan 12, 2022

Question 1

Which of the following would you find in a Sanger sequencing reaction but not in a polymerase chain reaction?
◦ DNA template
◦ DNA polymerase
◦ dNTPs
◦ ddNTPs
◦ DNA primer

Question 2

Why are leading and lagging strand primers removed rather than joined with Okazaki fragments?
◦ The primers do not hydrogen bond to the template strand correctly.
◦ They have to be removed for leading and lagging strand synthesis to begin.
◦ Primers are too short to efficiently bind to Okazaki fragments.
◦ They contain nucleotides with 2'OH groups, and are targeted for excision by DNA Polymerase.
◦ They lack the 3' OH that would permit them to be covalently linked to the Okazaki fragments.
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Marked as best answer by HudsonKB16 on Jan 12, 2022

sabina576

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Reply #1 on: Jan 12, 2022
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HudsonKB16

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Reply 2 on: Jan 12, 2022
Wow, this really help

pangili4

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Reply 3 on: Yesterday
Thanks for the timely response, appreciate it

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