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Author Question: Northern blot analysis is performed on mRNA isolated fromE. coliafter growth in a glucose-only ... (Read 91 times)

serike

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on: Jan 12, 2022

Question 1

In E. coli, the cya gene encodes adenylate cyclase. Will cells that are cya− be able to grow on medium that contains lactose as the sole carbon source? Why or why not?

Question 2

Northern blot analysis is performed on mRNA isolated from E. coli after growth in a glucose-only medium (lane 1) or a lactose-only medium (lane 2). The blots are hybridized with a probe for a portion of the lacZ sequence. Indicate the expected results for the following bacterial genotypes using dark, light, or no shading to indicate full, low, or no expression, respectively.
a. I+ P+ O+ Z+ Y+
b. I+ P- O+ Z+ Y+
c. I- P+ O+ Z+ Y+
d. I+ P+ OC Z+ Y+
e. I+ P+ O+ Z+ Y+ and a mutation of the CAP binding site preventing CAP-cAMP binding

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Marked as best answer by serike on Jan 12, 2022

bitingbit

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Reply #1 on: Jan 12, 2022
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serike

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Reply 2 on: Jan 12, 2022
Excellent

daiying98

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Reply 3 on: Yesterday
Great answer, keep it coming :)

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