This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Northern blot analysis is performed on mRNA isolated fromE. coliafter growth in a glucose-only ... (Read 51 times)

serike

  • Hero Member
  • *****
  • Posts: 542
on: Jan 12, 2022

Question 1

In E. coli, the cya gene encodes adenylate cyclase. Will cells that are cya− be able to grow on medium that contains lactose as the sole carbon source? Why or why not?

Question 2

Northern blot analysis is performed on mRNA isolated from E. coli after growth in a glucose-only medium (lane 1) or a lactose-only medium (lane 2). The blots are hybridized with a probe for a portion of the lacZ sequence. Indicate the expected results for the following bacterial genotypes using dark, light, or no shading to indicate full, low, or no expression, respectively.
a. I+ P+ O+ Z+ Y+
b. I+ P- O+ Z+ Y+
c. I- P+ O+ Z+ Y+
d. I+ P+ OC Z+ Y+
e. I+ P+ O+ Z+ Y+ and a mutation of the CAP binding site preventing CAP-cAMP binding

Genetic Analysis: An Integrated Approach ¦ Sanders, Bowman ¦ 3rd Edition
Buy Test-Bank


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by serike on Jan 12, 2022

bitingbit

  • Sr. Member
  • ****
  • Posts: 323
Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
Answer Preview
Only 60% of students answer this correctly


serike

  • Member
  • Posts: 542
Reply 2 on: Jan 12, 2022
Excellent

Missbam101

  • Member
  • Posts: 341
Reply 3 on: Yesterday
Wow, this really help

« previous next »
 

Did you know?

Medication errors are three times higher among children and infants than with adults.

Did you know?

Historic treatments for rheumatoid arthritis have included gold salts, acupuncture, a diet consisting of apples or rhubarb, nutmeg, nettles, bee venom, bracelets made of copper, prayer, rest, tooth extractions, fasting, honey, vitamins, insulin, snow collected on Christmas, magnets, and electric convulsion therapy.

Did you know?

Increased intake of vitamin D has been shown to reduce fractures up to 25% in older people.

Did you know?

After a vasectomy, it takes about 12 ejaculations to clear out sperm that were already beyond the blocked area.

Did you know?

In 2010, opiate painkllers, such as morphine, OxyContin®, and Vicodin®, were tied to almost 60% of drug overdose deaths.

Outline of pathways for glucose synthesis from the major gluconeogenic precursors Outline of pathways for glucose synthesis from the major gluconeogenic precursors
Kinetic analysis of spliceosome assembly Kinetic analysis of spliceosome assembly
Population growth and growth rate, 1950 - 2100 Population growth and growth rate, 1950 - 2100
Swot analysis Swot analysis
In the PCR amplification and analysis of one STR locus In the PCR amplification and analysis of one STR locus
One year ago, Christos bought 1000 units of the Dominion Aggressive Growth Fund at $20.35 per ... One year ago, Christos bought 1000 units of the Dominion Aggressive Growth Fund at $20.35 per ...
Video: Effect of the environment on coat color in the Himalayan rabbit Animation

For a complete list of videos, visit our video library