Author Question: Northern blot analysis is performed on mRNA isolated fromE. coliafter growth in a glucose-only ... (Read 50 times)

serike · **on:** Jan 12, 2022

Question 1

In E. coli, the cya gene encodes adenylate cyclase. Will cells that are cya− be able to grow on medium that contains lactose as the sole carbon source? Why or why not?

Question 2

Northern blot analysis is performed on mRNA isolated from E. coli after growth in a glucose-only medium (lane 1) or a lactose-only medium (lane 2). The blots are hybridized with a probe for a portion of the lacZ sequence. Indicate the expected results for the following bacterial genotypes using dark, light, or no shading to indicate full, low, or no expression, respectively.
a. I+ P+ O+ Z+ Y+
b. I+ P- O+ Z+ Y+
c. I- P+ O+ Z+ Y+
d. I+ P+ OC Z+ Y+
e. I+ P+ O+ Z+ Y+ and a mutation of the CAP binding site preventing CAP-cAMP binding

bitingbit · **Reply #1 on:** Jan 12, 2022

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