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Author Question: Northern blot analysis is performed on mRNA isolated fromE. coliafter growth in a glucose-only ... (Read 40 times)

serike

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on: Jan 12, 2022

Question 1

In E. coli, the cya gene encodes adenylate cyclase. Will cells that are cya− be able to grow on medium that contains lactose as the sole carbon source? Why or why not?

Question 2

Northern blot analysis is performed on mRNA isolated from E. coli after growth in a glucose-only medium (lane 1) or a lactose-only medium (lane 2). The blots are hybridized with a probe for a portion of the lacZ sequence. Indicate the expected results for the following bacterial genotypes using dark, light, or no shading to indicate full, low, or no expression, respectively.
a. I+ P+ O+ Z+ Y+
b. I+ P- O+ Z+ Y+
c. I- P+ O+ Z+ Y+
d. I+ P+ OC Z+ Y+
e. I+ P+ O+ Z+ Y+ and a mutation of the CAP binding site preventing CAP-cAMP binding

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Marked as best answer by serike on Jan 12, 2022

bitingbit

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Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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serike

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Reply 2 on: Jan 12, 2022
Gracias!

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Reply 3 on: Yesterday
Great answer, keep it coming :)

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