This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: In producing a recombinant plasmid to be used to clone a given donor insert, it is possible to cut ... (Read 68 times)

Wadzanai

  • Hero Member
  • *****
  • Posts: 528
on: Jan 12, 2022
In producing a recombinant plasmid to be used to clone a given donor insert, it is possible to cut both the donor and plasmid with the same restriction enzyme, resulting in complementary sticky ends. Assuming plenty of plasmid DNA is available, why is further selection necessary before the introduction of the plasmid into a cellular system?
◦ Contamination will have introduced other donor inserts.
◦ Some donor inserts will be single stranded and deteriorate.
◦ Some donor inserts will be sensitive to particular antibiotics.
◦ Some donor pieces will remain uninserted.
◦ Some donor strands will be inserted with an incorrect orientation.
Genetic Analysis: An Integrated Approach ¦ Sanders, Bowman ¦ 3rd Edition
Buy Test-Bank


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by Wadzanai on Jan 12, 2022

akpaschal

  • Sr. Member
  • ****
  • Posts: 342
Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
Answer Preview
Only 56% of students answer this correctly


Wadzanai

  • Member
  • Posts: 528
Reply 2 on: Jan 12, 2022
Gracias!

bimper21

  • Member
  • Posts: 309
Reply 3 on: Yesterday
Wow, this really help

« previous next »
 

Did you know?

According to the FDA, adverse drug events harmed or killed approximately 1,200,000 people in the United States in the year 2015.

Did you know?

Not getting enough sleep can greatly weaken the immune system. Lack of sleep makes you more likely to catch a cold, or more difficult to fight off an infection.

Did you know?

Barbituric acid, the base material of barbiturates, was first synthesized in 1863 by Adolph von Bayer. His company later went on to synthesize aspirin for the first time, and Bayer aspirin is still a popular brand today.

Did you know?

Atropine, along with scopolamine and hyoscyamine, is found in the Datura stramonium plant, which gives hallucinogenic effects and is also known as locoweed.

Did you know?

Normal urine is sterile. It contains fluids, salts, and waste products. It is free of bacteria, viruses, and fungi.

Workers at a Nike factory in Indonesia insert soles into sneakers for fourteen cents an hour. Workers at a Nike factory in Indonesia insert soles into sneakers for fourteen cents an hour.
Flow scattergrams showing results on the top set from a healthy donor and on the bottom set from a ... Flow scattergrams showing results on the top set from a healthy donor and on the bottom set from a ...
Recombinant Vaccines - protein subunit Recombinant Vaccines - protein subunit
DNA inserted into multiple cloning site of a plasmid disrupts DNA inserted into multiple cloning site of a plasmid disrupts
Plasmid cloning vectors Plasmid cloning vectors
Mutation of Raly and Agouti producing yellow coat Mutation of Raly and Agouti producing yellow coat
Video: Embryonic development - Week 3

For a complete list of videos, visit our video library