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Author Question: In producing a recombinant plasmid to be used to clone a given donor insert, it is possible to cut ... (Read 26 times)

Wadzanai

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on: Jan 12, 2022
In producing a recombinant plasmid to be used to clone a given donor insert, it is possible to cut both the donor and plasmid with the same restriction enzyme, resulting in complementary sticky ends. Assuming plenty of plasmid DNA is available, why is further selection necessary before the introduction of the plasmid into a cellular system?
◦ Contamination will have introduced other donor inserts.
◦ Some donor inserts will be single stranded and deteriorate.
◦ Some donor inserts will be sensitive to particular antibiotics.
◦ Some donor pieces will remain uninserted.
◦ Some donor strands will be inserted with an incorrect orientation.
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Marked as best answer by Wadzanai on Jan 12, 2022

akpaschal

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Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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Wadzanai

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Reply 2 on: Jan 12, 2022
Wow, this really help

vickybb89

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Reply 3 on: Yesterday
Thanks for the timely response, appreciate it

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