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Author Question: Which of the following best lists the correct order of the steps taken to produce human insulin ... (Read 41 times)

jho37

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on: Jan 12, 2022
Which of the following best lists the correct order of the steps taken to produce human insulin in E.coli in the 1970s?

I.Each insulin chain was cloned into an expression vector as a continuation of the lacZ reading 
frame, resulting in a fusion protein for each chain.
II.Disulfide bonds were induced to form between the cysteine residues of the purified insulin A 
and B chains.
III.Both human insulin genes were reverse translated to create synthetic genes.
IV.EcoRI and BamHI restriction sites were added to the ends of the DNA to facilitate directional 
cloning of each into a separate plasmid vector.

◦ I, II, III, IV
◦ III, IV, I, II
◦ IV, III, I, II
◦ III, I, II, IV
◦ I, III, IV, II
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Marked as best answer by jho37 on Jan 12, 2022

ririgirl15

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Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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jho37

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Reply 2 on: Jan 12, 2022
:D TYSM

amandalm

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Reply 3 on: Yesterday
Thanks for the timely response, appreciate it

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