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Author Question: What is the most logical sequence of steps for splicing foreign DNA into a plasm (Read 673 times)

camila

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on: Jul 3, 2013
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I.   Transform bacteria with a recombinant DNA molecule.
II.   Cut the plasmid DNA using restriction enzymes.
III.   Extract plasmid DNA from bacterial cells.
IV.   Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
V.   Use ligase to seal plasmid DNA to nonplasmid DNA.
A) I, II, IV, III, V
B) II, III, V, IV, I
C) III, II, IV, V, I
D) III, IV, V, I, II
E) IV, V, I, II, III


Jesse_J

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Reply #1 on: Jul 4, 2013
It's definitely C, good luck with the rest.


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camila

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Reply #2 on: Jul 9, 2013
Thanks :D


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