A 17-year-old female exhibits signs of disseminated gonorrhea. A total of 6 mL of blood was drawn and divided between two bottles of blood culture broth with SPS, each containing a volume of 40 mL.
While her endocervical culture grows Neisseria gonorrhoeae, her blood cultures remain negative. What is the explanation for the lack of growth in the blood culture vials?
A) Blood culture bottles with heparin should have used for blood collection.
B) Too little blood was drawn in the vials and organism growth was inhibited.
C) Blood culture bottles with resin beads should have been used for blood collection.
D) Sucrose was not added and the organisms need a hyperosmotic solution to survive.
Question 2
Cell culture maintenance medium is used to
A) encourage viral proliferation. B) prevent contamination with Mycoplasma spp.
C) sustain cells in a steady metabolic state. D) promote rapid cell growth.
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