What procedure is used to quantitate a factor VIII inhibitor? ...

[DyllonKazuo]

What procedure is used to quantitate a factor VIII inhibitor?
 
  a. Bethesda assay
  b. Reptilase time
  c. Factor VIII concentration
  d. VWF assay

Question 2

A 67-year-old woman arrives at the emergency department with acute bleeding into the back of the throat. A coagulation screen is ordered with the following results:
 
  Test Result Reference Range 1:1 Mix with Normal Plasma
  Prothrombin time (PT 12 seconds 11-15 seconds
  Partial thromboplastin time (PTT) 57 seconds 25-37 seconds 36 seconds (immediate)
  Fibrinogen 450 mg/dL 150-400 mg/dL
 
  What would be a reasonable next step to perform?
 
  a. Perform factor IX assay.
  b. Perform factor VII assay.
  c. Incubate the 1:1 mix at 37 C for 1 hour and then repeat the PTT.
  d. Perform TT.

[katheyjon]

Answer to Question 1

ANS: A
The Bethesda assay is used to quantitate a factor VIII inhibitor.

Answer to Question 2

ANS: C
Factor VIII autoantibodies are usually time and temperature dependent. Thus the 1:1 mix must be incubated at 37 C for 1 to 2 hours to give the antibody time to react with the factor VIII added by the normal plasma. Prolongation of the PTT on this incubated 1:1 mix indicates that an antibody to VIII is present. A factor VIII assay, when performed, will show a low factor VIII (less than 30).

[DyllonKazuo]

Thanks for the timely response, appreciate it

[cici]

TYSM