Author Question: Which automatic hematology analyzer performs the white count (by both optical scatter and electrical ... (Read 53 times)

debasdf · **on:** Jun 25, 2018

Which automatic hematology analyzer performs the white count (by both optical scatter and electrical impedance) and then compares the two as an internal quality control check?

a. Sysmex 9000/9500
b. Coulter LH750
c. CELL-DYN 4000
d. Advia 2100

Question 2

An automated hematology analyzer is used to evaluate a blood specimen. Which of the following, coming off the instrument, would require performance of a manual white cell differential as opposed to blood film review?

a. Flagged data
b. Suppression of automated differential data
c. Abnormal red cell indices
d. A slightly elevated platelet count

Laurenleakan · **Reply #1 on:** Jun 25, 2018

Answer to Question 1

ANS: C
The CELL-DYN 4000 performs the white count using optical scatter (WOC) and electrical impedance (WIC). Software compares the two as an internal quality control check. When the WOC and the WIC differ, an algorithm selects the most appropriate value for the reported white cell count. Newborn samples, which contain a high concentration of fetal hemoglobin, are an example of when a discrepancy might exist between the two. The soft lysing agent used in the WOC channel is unable to completely lyse the red cells, raising the WOC count compared with the WIC.

Answer to Question 2

ANS: B
When internal instrument checks fail or cast doubt on the data, the automated differential data are suppressed. This means that a manual differential should be performed. Flagged data may be released after appropriate blood film review, as dictated by the specific laboratory policy.