Author Question: Hb A2 and Hb F can be quantified by: a. Performing a Brilliant cresyl blue stain and counting the ... (Read 72 times)

Evvie72 · **on:** Jun 25, 2018

Hb A2 and Hb F can be quantified by:

a. Performing a Brilliant cresyl blue stain and counting the number of positive cells
b. Performing high-performance liquid chromatography
c. Performing cellulose acetate hemoglobin electrophoresis
d. Performing citrate agar hemoglobin electrophoresis

Question 2

Patients who are heterozygous for Hb Lepore have a clinical course:

a. That is completely benign
b. Similar to that of b-thalassemia minor
c. Similar to that of a-thalassemia minor (one gene deletion)
d. Similar to that of b-thalassemia major

spencer.martell · **Reply #1 on:** Jun 25, 2018

Answer to Question 1

ANS: B
High-performance liquid chromatography can separate and quantify Hb A2 and Hb F, which is important to do when working up patients with possible thalassemic syndromes. None of the other methods quantify them.

Answer to Question 2

ANS: B
Hb Lepore is a d-b fusion globin chain with decreased synthesis of the chain. Heterozygotes have a clinical course similar to that of b-thalassemia minor.