« previous next »

Author Question: What is the total volume of one side of a hemocytometer if the ruled area is 3 3 mm and the depth ... (Read 31 times)

futuristic

  • Hero Member
  • *****
  • Posts: 638
on: Jun 25, 2018
What is the total volume of one side of a hemocytometer if the ruled area is 3  3 mm and the depth is 0.1 mm?
 
  a. 0.3 mm3
  b. 0.9 mm3
  c. 1.0 mm3
  d. 9 mm3

Question 2

Which of the following would cause a manual cell count to be falsely high?
 
  a. Unopette not mixed before charging the hemocytometer
  b. Not wiping excess blood from outside of pipette when filling
  c. Using a smaller volume pipette than that specified by the Unopette system
  d. Pulling blood out of the pipette when wiping away excess on the outside


kishoreddi

  • Sr. Member
  • ****
  • Posts: 329
    • Your online Help in Biology and chemistry
Reply #1 on: Jun 25, 2018
Answer to Question 1

ANS: B
Volume = area  depth = (3  3)  0.1 = 0.9 mm3

Answer to Question 2

ANS: B
Blood left on the outside of the pipette will add to the blood in the pipette when the pipette tip is placed in the Unopette reservoir. This will increase and invalidate the cell count (falsely high). If the Unopette was not mixed before charging, then the count would most likely be low. Pulling blood out of the pipette when wiping away the excess on the outside will falsely lower the cell count because less blood will be present. Using a smaller volume pipette than required will lower the count, again because less blood will be present.


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
« previous next »
 

Did you know?

Acute bronchitis is an inflammation of the breathing tubes (bronchi), which causes increased mucus production and other changes. It is usually caused by bacteria or viruses, can be serious in people who have pulmonary or cardiac diseases, and can lead to pneumonia.

Did you know?

People with alcoholism are at a much greater risk of malnutrition than are other people and usually exhibit low levels of most vitamins (especially folic acid). This is because alcohol often takes the place of 50% of their daily intake of calories, with little nutritional value contained in it.

Did you know?

Since 1988, the CDC has reported a 99% reduction in bacterial meningitis caused by Haemophilus influenzae, due to the introduction of the vaccine against it.

Did you know?

Carbamazepine can interfere with the results of home pregnancy tests. If you are taking carbamazepine, do not try to test for pregnancy at home.

Did you know?

It is important to read food labels and choose foods with low cholesterol and saturated trans fat. You should limit saturated fat to no higher than 6% of daily calories.

Replica of an Asian elephant's molar, showing upper side. Replica of an Asian elephant's molar, showing upper side.
Emphysema. (a) Illustration comparing normal lungs and emphysemic lungs. The inserts illustrate how Emphysema. (a) Illustration comparing normal lungs and emphysemic lungs. The inserts illustrate how
Effects of Living in Total Darkness on Monoaminergic Systems Effects of Living in Total Darkness on Monoaminergic Systems
Triceps and Side Stretch With Arm Overhead. Triceps and Side Stretch With Arm Overhead.
The inguinal area. Take great care when applying deep pressure in this region The inguinal area. Take great care when applying deep pressure in this region
Stand at the side facing the client. Apply L stroke to both sides of the neck with flat fingers ... Stand at the side facing the client. Apply L stroke to both sides of the neck with flat fingers ...
Video: Energy sources - types

For a complete list of videos, visit our video library