Author Question: What is the total volume of one side of a hemocytometer if the ruled area is 3 3 mm and the depth ... (Read 28 times)

futuristic

  • Hero Member
  • *****
  • Posts: 638
What is the total volume of one side of a hemocytometer if the ruled area is 3  3 mm and the depth is 0.1 mm?
 
  a. 0.3 mm3
  b. 0.9 mm3
  c. 1.0 mm3
  d. 9 mm3

Question 2

Which of the following would cause a manual cell count to be falsely high?
 
  a. Unopette not mixed before charging the hemocytometer
  b. Not wiping excess blood from outside of pipette when filling
  c. Using a smaller volume pipette than that specified by the Unopette system
  d. Pulling blood out of the pipette when wiping away excess on the outside



kishoreddi

  • Sr. Member
  • ****
  • Posts: 329
    • Your online Help in Biology and chemistry
Answer to Question 1

ANS: B
Volume = area  depth = (3  3)  0.1 = 0.9 mm3

Answer to Question 2

ANS: B
Blood left on the outside of the pipette will add to the blood in the pipette when the pipette tip is placed in the Unopette reservoir. This will increase and invalidate the cell count (falsely high). If the Unopette was not mixed before charging, then the count would most likely be low. Pulling blood out of the pipette when wiping away the excess on the outside will falsely lower the cell count because less blood will be present. Using a smaller volume pipette than required will lower the count, again because less blood will be present.



Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
 

Did you know?

Chronic marijuana use can damage the white blood cells and reduce the immune system's ability to respond to disease by as much as 40%. Without a strong immune system, the body is vulnerable to all kinds of degenerative and infectious diseases.

Did you know?

Aspirin is the most widely used drug in the world. It has even been recognized as such by the Guinness Book of World Records.

Did you know?

Patients who cannot swallow may receive nutrition via a parenteral route—usually, a catheter is inserted through the chest into a large vein going into the heart.

Did you know?

Giardia is one of the most common intestinal parasites worldwide, and infects up to 20% of the world population, mostly in poorer countries with inadequate sanitation. Infections are most common in children, though chronic Giardia is more common in adults.

Did you know?

All patients with hyperparathyroidism will develop osteoporosis. The parathyroid glands maintain blood calcium within the normal range. All patients with this disease will continue to lose calcium from their bones every day, and there is no way to prevent the development of osteoporosis as a result.

For a complete list of videos, visit our video library