« previous next »

Author Question: Class IV G6PD enzymes include which of the following? a. G6PD-Mediterranean b. G6PD-B and ... (Read 55 times)

jenna1

  • Hero Member
  • *****
  • Posts: 568
on: Jun 25, 2018
Class IV G6PD enzymes include which of the following?
 
  a. G6PD-Mediterranean
  b. G6PD-B and G6PD-A+
  c. G6PD-Iowa
  d. G6PD-Canton

Question 2

What are the principles of the following tests?
 
  a. Fluorescent spot test
  b. Dye reduction test
  c. Ascorbate cyanide test
  d. Rate reduction test
 
  What will be an ideal response?


sultansheikh

  • Sr. Member
  • ****
  • Posts: 335
Reply #1 on: Jun 25, 2018
Answer to Question 1

Correct Answer: B

Answer to Question 2

Answer: Fluorescent spot test: Whole blood is added to a mixture of G6P, NADP, and saponin. G6PD enzyme metabolizes G6P and produces NADPH (which fluoresces). NADP does not. Lack of fluorescence indicates G6PD deficiency.
Dye reduction test: similar to fluorescent spot test except for indicator: blue color to colorless  presence of G6PD; blue color remains  absence of G6PD
Ascorbate cyanide test: nonspecific test. Tests the ability of RBC catalase to inhibit hydrogen peroxide formation. G6PD-deficient patients allow hydrogen peroxide to form, which promotes the formation of methemoglobin. The presence of methemoglobin turns the solution brown.
Rate reduction test: Measures rate of conversion of NADP to NADPH via spectrophotometry at 340 nm.


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
« previous next »
 

Did you know?

There used to be a metric calendar, as well as metric clocks. The metric calendar, or "French Republican Calendar" divided the year into 12 months, but each month was divided into three 10-day weeks. Each day had 10 decimal hours. Each hour had 100 decimal minutes. Due to lack of popularity, the metric clocks and calendars were ended in 1795, three years after they had been first marketed.

Did you know?

Individuals are never “cured” of addictions. Instead, they learn how to manage their disease to lead healthy, balanced lives.

Did you know?

Every 10 seconds, a person in the United States goes to the emergency room complaining of head pain. About 1.2 million visits are for acute migraine attacks.

Did you know?

Before a vaccine is licensed in the USA, the Food and Drug Administration (FDA) reviews it for safety and effectiveness. The CDC then reviews all studies again, as well as the American Academy of Pediatrics and the American Academy of Family Physicians. Every lot of vaccine is tested before administration to the public, and the FDA regularly inspects vaccine manufacturers' facilities.

Did you know?

According to the National Institute of Environmental Health Sciences, lung disease is the third leading killer in the United States, responsible for one in seven deaths. It is the leading cause of death among infants under the age of one year.

A Mediterranean woodland in California shown during the cool moist season, when the herbaceous veget A Mediterranean woodland in California shown during the cool moist season, when the herbaceous veget
John Lennon - Working Class Hero John Lennon - Working Class Hero
A miner greets the president. Franklin’s “first-class temperament” compensated for his ... A miner greets the president. Franklin’s “first-class temperament” compensated for his ...
Five Factors of Personality and the Traits they Include Five Factors of Personality and the Traits they Include
Enzymes substrate specificity! Enzymes substrate specificity!
Noncoding regions include all the 5′UTR, 3′UTR and other noncoding RNA regions. UTR = ... Noncoding regions include all the 5′UTR, 3′UTR and other noncoding RNA regions. UTR = ...
Video: Pericarditis

For a complete list of videos, visit our video library