Author Question: Which of the following physical properties are required for an autoclave to sterilize biohazardous ... (Read 44 times)

Yolanda · **on:** Jul 16, 2018

Which of the following physical properties are required for an autoclave to sterilize biohazardous material?

1. Dry heat
2. A vacuum
3. 100 humidity
4. Increased air pressure
a. 2 and 3 c. 1, 2, and 4
b. 1 and 3 d. 2, 3, and 4

Question 2

A 1.25 solution of acetic acid has been shown to be an effective bactericidal agent against:

a. Staphylococcus au-reus c. Micobacterium tu-berculosis
b. Pseudomonas aeru-ginosa d. Streptococcus pneumoniae

joneynes · **Reply #1 on:** Jul 16, 2018

Answer to Question 1

ANS: D
Air is evacuated, moisture is added (100 humidity), and the pressure inside the autoclave is raised to 15 to 20 lb-force per square inch gauge (psig). Air is evacuated from the chamber be-cause residual air prolongs the penetration time of steam, thus increasing the total autoclave cycle time. Pressure is used to raise the temperature of the steam, which is critical because the amount of time required to achieve sterilization depends on the temperature inside of the autoclave. Dry heat is not used during autoclaving.

Answer to Question 2

ANS: B
Pseudomonas aeruginosa is killed by 1.25 acetic acid or one part 5 white household vinegar and three parts water. Pseudomonas aeruginosa transmission is through indirect contact via fom-ites, such as those on clothing, surgical bandages, and especially equipment. It is the most com-mon respiratory pathogen in patients with cystic fibrosis and is encountered in patients with chronic obstructive pulmonary disease.