After reading the paragraph below, answer the questions that follow.
Thin-layer chromatography is a method that can be used to separate molecules by their polarity. A mixture of molecules in a nonpolar liquid (the mobile phase) is added to the bottom of a piece of adsorbent material (the stationary phase) and allowed to migrate up the material. More-polar molecules interact more strongly with the stationary phase and thus migrate less. Less-polar molecules interact less strongly with the stationary phase and thus migrate more. Below are sample data for four different molecules from a thin-layer chromatography experiment. [Art: custom PowerPoint figure created by the author]
What can you conclude from these data?
◦ Molecule D is more polar than molecule A.
◦ Molecule A is an ion.
◦ Molecule C is the most polar molecule.
◦ Molecule B contains many C-H bonds.
A trilobite fragment (T) in a thin-section of an Ordovician limestone; E=echinoderm; scale bar is 2
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