After reading the paragraph below, answer the questions that follow.
Studies of the endomembrane system often involve the use of a protein that can emit a green fluorescence (glow). A researcher wants to make a video of cell behavior, so she initially labels the outer nuclear envelope of a cell with the fluorescent tag and records a video for several hours. Later, she sees that the tag is part of a transport vesicle very close to the plasma membrane.
What likely happened during the intervening time?
◦ The endoplasmic reticulum engulfed the tagged protein and released it in a transport vacuole.
◦ Material from the nucleus, including the tag, was being secreted outside the cell.
◦ The outer membrane of the nucleus broke off and moved the fluorescent tag to the Golgi.
◦ The tag was seen in the Golgi before being seen in a transport vacuole.
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