After reading the paragraph below, answer the questions that follow.
Lipase is an enzyme released by the pancreas that digests fats in the small intestine; however, lipase is also found in bacteria. Researchers examined lipase function in two species of bacteria, species X and species Y. Data regarding the activity of lipase as a function of temperature are shown below for the lipase from bacteria X (white circles) and the lipase from bacteria Y (black circles). Use this information to answer the following three questions.
Lipase activity as a function of temperature.
What can you conclude from data in the figure?
◦ The lipase from bacteria X is insensitive to changes in pH.
◦ Bacteria X likely lives in an environment that undergoes large changes in temperature.
◦ Only the lipase from bacteria Y denatures at high temperatures.
◦ The lipase from bacteria Y is more active than the lipase from bacteria X at 20°C.
Step 2 of gene cloning: The actions of a restriction enzyme and DNA ligase to produce a recombinant
Influence of substrate concentration on the rate of an enzyme-catalyzed reaction.
Enzyme
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