This topic contains a solution. Click here to go to the answer

Author Question: After reading the paragraph below, answer the questions that follow.While DNA microarrays can be ... (Read 139 times)

luminitza

  • Hero Member
  • *****
  • Posts: 555
After reading the paragraph below, answer the questions that follow.

While DNA microarrays can be used to determine whether or not a gene is expressed in a cell, quantitative polymerase chain reaction (qPCR) can be used to specifically quantify the extent of gene expression in cells and tissues. To perform qPCR, mRNA is extracted from cells and reverse transcribed to complementary DNA (cDNA). Using fluorescent DNA molecules that recognize specific gene sequences and DNA polymerase, copies of the gene sequence of interest are made. After the copies are made, a fluorescent detector measures the intensity of the fluorescent signal, which is proportional to the number of mRNA transcripts in the cell and corresponds to the level of gene expression in the cell.


In a muscle cell, the contractile protein myosin is present in larger quantities than the structural protein actin. Which of the following qPCR results would support this observation?
◦ The fluorescent signal for myosin is higher than that of actin.
◦ The fluorescent signals for myosin and actin are equal.
◦ The fluorescent signal for myosin is large and is zero for actin.
◦ The fluorescent signal for actin is higher than that of myosin.


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by luminitza on Jul 15, 2019

canderson530

  • Sr. Member
  • ****
  • Posts: 317
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
Answer Preview
Only 25% of students answer this correctly





 

Did you know?

Every flu season is different, and even healthy people can get extremely sick from the flu, as well as spread it to others. The flu season can begin as early as October and last as late as May. Every person over six months of age should get an annual flu vaccine. The vaccine cannot cause you to get influenza, but in some seasons, may not be completely able to prevent you from acquiring influenza due to changes in causative viruses. The viruses in the flu shot are killed—there is no way they can give you the flu. Minor side effects include soreness, redness, or swelling where the shot was given. It is possible to develop a slight fever, and body aches, but these are simply signs that the body is responding to the vaccine and making itself ready to fight off the influenza virus should you come in contact with it.

Did you know?

Oliver Wendell Holmes is credited with introducing the words "anesthesia" and "anesthetic" into the English language in 1846.

Did you know?

The people with the highest levels of LDL are Mexican American males and non-Hispanic black females.

Did you know?

Increased intake of vitamin D has been shown to reduce fractures up to 25% in older people.

Did you know?

As many as 28% of hospitalized patients requiring mechanical ventilators to help them breathe (for more than 48 hours) will develop ventilator-associated pneumonia. Current therapy involves intravenous antibiotics, but new antibiotics that can be inhaled (and more directly treat the infection) are being developed.

For a complete list of videos, visit our video library