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Author Question: Some restriction enzymes do not leave sticky ends when cutting DNA molecules; rather, they cut a ... (Read 177 times)

mydiamond

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on: Jul 15, 2019
Some restriction enzymes do not leave sticky ends when cutting DNA molecules; rather, they cut a restriction site down the middle and leave "blunt-ended" DNA molecules, which do not have any single-stranded nucleotide extensions. Which of the following is not a likely outcome when trying to insert a gene into a plasmid when both have been cut with the same "blunt-ended" restriction enzyme?
◦ The gene might be inserted into the plasmid multiple times in a row.
◦ The gene might insert into the plasmid in the proper (forward) orientation.
◦ The gene might insert into the plasmid in the wrong (backward) orientation.
◦ The gene might be inserted into the plasmid by forming hydrogen bonds.
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Marked as best answer by mydiamond on Jul 15, 2019

AmberC1996

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Reply #1 on: Jul 15, 2019
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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mydiamond

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Reply #2 on: Jul 15, 2019
TY


AmberC1996

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Reply #3 on: Jul 15, 2019
You're welcome


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