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Author Question: Suppose a researcher is interested in using the CRISPR-Cas9 system to knock out the ... (Read 414 times)

codyclark

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Suppose a researcher is interested in using the CRISPR-Cas9 system to knock out the phosphofructokinase gene of a rat skin cell that codes for an enzyme that is critical for glycolysis to occur. The researcher added the Cas9 enzyme to the cell but forgot to add the guide RNA. What result do you think the researcher will observe?
◦ Glycolysis will proceed normally in the cell.
◦ Cas9 will specifically cut the phosphofructokinase gene.
◦ The phosphofructokinase enzyme will be expressed but will have lower activity.
◦ There will be mutations in the phosphofructokinase gene.


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Marked as best answer by codyclark on Jul 15, 2019

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