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Author Question: Before transferring DNA from a gel to the membrane in Southern blotting, the DNA must be denatured ... (Read 93 times)

serike

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on: Jan 12, 2022

Question 1

You identify a new unicellular organism with multiple chromosomes organized by proteins within the cell's nucleus. Into which of the three domains of life might this organism fit?
◦ Archaea
◦ Bacteria
◦ Eukarya
◦ Archaea or Bacteria
◦ Archaea or Eukarya

Question 2

Before transferring DNA from a gel to the membrane in Southern blotting, the DNA must be denatured (usually by soaking the gel in NaOH). Why is this step necessary?
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Marked as best answer by serike on Jan 12, 2022

essyface1

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Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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serike

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Reply 2 on: Jan 12, 2022
YES! Correct, THANKS for helping me on my review

amcvicar

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Reply 3 on: Yesterday
Great answer, keep it coming :)

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