A lab technician has four strains of bacteria. Although he forgot to label them, he knows that they include one F
+ strain, one Hfr strain, one F' strain, and one F
- strain. He observed the following results after plating these strains on different media. Which of the following plates can be used to identify the F- strain?
strain | his | glu | cys | leu | str |
F+ | + | + | + | - | + |
Hfr | + | + | + | + | - |
F' | + | + | + | + | - |
F- | - | - | - | + | + |
◦ complete media
◦ minimal media with histidine, glutamate, and cysteine
◦ minimal media with histidine, glutamate, cysteine, and streptomycin
◦ minimal media with streptomycin
◦ minimal media with leucine and streptomycin