Question 1
The proofreading activity of a DNA polymerase is primarily provided by its:
◦ 5'-3' exonuclease activity.
◦ 3'-5' exonuclease activity.
◦ 5'-3' polymerase activity.
◦ 3'-5' polymerase activity.
◦ 5'-3' endonuclease activity.
Question 2
The required features for DNA ligase to seal a nick in double-stranded DNA are:
◦ 5' and 3' phosphate ends adjacent to each and paired with another strand of DNA.
◦ 5' and 3' OH ends adjacent to each other and paired with another strand of DNA.
◦ a 5' phosphate and a 3' OH end adjacent to each other and paired with another strand of DNA.
◦ a 5' OH and a 3' phosphate end adjacent to each other and paired with another strand of DNA.
◦ an AMP only.