Question 1
Replication of phage φX174 is initiated by:
◦ a random nick in the circular genome.
◦ multiple random nicks in the genome.
◦ bidirectional replication from a specific origin.
◦ bidirectional replication from multiple origins.
◦ a site-specific nick in the circular genome.
Question 2
T-DNA is transferred from the bacterial cell to the plant cell as a:
◦ single-stranded DNA beginning from a nick at the leftward T-DNA repeat.
◦ single-stranded DNA beginning from a nick at the rightward T-DNA repeat.
◦ single-stranded DNA beginning from simultaneous nicks at the leftward and rightward T-DNA repeats.
◦ double-stranded DNA beginning from a double-strand cut at the leftward T-DNA repeat.
◦ double-stranded DNA beginning from a double-strand cut at the rightward T-DNA repeat.