The first step in translation initiation in bacteria is:
◦ association of initiator tRNA with the 30S ribosomal subunit.
◦ association of mRNA with the 30S ribosomal subunit.
◦ 50S subunit binding to the 30S ribosomal subunit.
◦ association of IF-1 and IF-3 with the 30S ribosomal subunit.
◦ stabilize free 50S ribosomal subunits to facilitate assembly into mature ribosome.