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Author Question: How to amplify DNA fragments with given sequences? (Read 865 times)

tingc95

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on: Oct 26, 2014
I have a Bio test on Monday and I've been stuck on the short answer problems like this one:
Sequence #1;
5'ATCGATGTGCTATACATGT TAACAC3'
3'TAGCTACACGATATGTACA CTTGTG5'
Sequence #2:
5'ATCGATGTGCTAAACATGT TAACAC3'
3'TAGCTACACGATATGTACA CTTGTG5'

Q1: Give the sequence and polarity of two DNA primers 6 nucleotides long that would allow you to amplify the entire fragment?
Q2: Assuming the bottom strand is the DNA template used for transcription, what is the amino acid sequence of the protein that is produced by Sequence #1.

I have no idea how to do those questions, I've tried finding similar problems in the textbook, but to no success. I appreciate any help I can get, thank you!


Celeste

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  • Posts: 588
Reply #1 on: May 22, 2019
This document should help :)


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