« previous next »

Author Question: How to amplify DNA fragments with given sequences? (Read 877 times)

tingc95

  • Hero Member
  • *****
  • Posts: 556
on: Oct 26, 2014
I have a Bio test on Monday and I've been stuck on the short answer problems like this one:
Sequence #1;
5'ATCGATGTGCTATACATGT TAACAC3'
3'TAGCTACACGATATGTACA CTTGTG5'
Sequence #2:
5'ATCGATGTGCTAAACATGT TAACAC3'
3'TAGCTACACGATATGTACA CTTGTG5'

Q1: Give the sequence and polarity of two DNA primers 6 nucleotides long that would allow you to amplify the entire fragment?
Q2: Assuming the bottom strand is the DNA template used for transcription, what is the amino acid sequence of the protein that is produced by Sequence #1.

I have no idea how to do those questions, I've tried finding similar problems in the textbook, but to no success. I appreciate any help I can get, thank you!


Celeste

  • Hero Member
  • Posts: 588
Reply #1 on: May 22, 2019
This document should help :)


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
« previous next »
 

Did you know?

In 1844, Charles Goodyear obtained the first patent for a rubber condom.

Did you know?

Amoebae are the simplest type of protozoans, and are characterized by a feeding and dividing trophozoite stage that moves by temporary extensions called pseudopodia or false feet.

Did you know?

Disorders that may affect pharmacodynamics include genetic mutations, malnutrition, thyrotoxicosis, myasthenia gravis, Parkinson's disease, and certain forms of insulin-resistant diabetes mellitus.

Did you know?

Approximately one in three babies in the United States is now delivered by cesarean section. The number of cesarean sections in the United States has risen 46% since 1996.

Did you know?

Recent studies have shown that the number of medication errors increases in relation to the number of orders that are verified per pharmacist, per work shift.

The organization of nucleotide sequences in bacterial chromosomal DNA. The organization of nucleotide sequences in bacterial chromosomal DNA.
Telomere sequences at the end of a human chromosome Telomere sequences at the end of a human chromosome
A common organization of sequences for the promoter of a eukaryotic structural gene A common organization of sequences for the promoter of a eukaryotic structural gene
Forest fragments left by clear-cutting forest from the surrounding landscape have very different phy Forest fragments left by clear-cutting forest from the surrounding landscape have very different phy
Archaeal promoter consensus sequences Archaeal promoter consensus sequences
Removal and replacement of RNA primer nucleotides and ligation of Okazaki fragments in E. coli Removal and replacement of RNA primer nucleotides and ligation of Okazaki fragments in E. coli
Video: Hormonal feedback loop components

For a complete list of videos, visit our video library