« previous next »

Author Question: How to amplify DNA fragments with given sequences? (Read 834 times)

tingc95

  • Hero Member
  • *****
  • Posts: 556
on: Oct 26, 2014
I have a Bio test on Monday and I've been stuck on the short answer problems like this one:
Sequence #1;
5'ATCGATGTGCTATACATGT TAACAC3'
3'TAGCTACACGATATGTACA CTTGTG5'
Sequence #2:
5'ATCGATGTGCTAAACATGT TAACAC3'
3'TAGCTACACGATATGTACA CTTGTG5'

Q1: Give the sequence and polarity of two DNA primers 6 nucleotides long that would allow you to amplify the entire fragment?
Q2: Assuming the bottom strand is the DNA template used for transcription, what is the amino acid sequence of the protein that is produced by Sequence #1.

I have no idea how to do those questions, I've tried finding similar problems in the textbook, but to no success. I appreciate any help I can get, thank you!


Celeste

  • Hero Member
  • Posts: 588
Reply #1 on: May 22, 2019
This document should help :)


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
« previous next »
 

Did you know?

There are immediate benefits of chiropractic adjustments that are visible via magnetic resonance imaging (MRI). It shows that spinal manipulation therapy is effective in decreasing pain and increasing the gaps between the vertebrae, reducing pressure that leads to pain.

Did you know?

Bisphosphonates were first developed in the nineteenth century. They were first investigated for use in disorders of bone metabolism in the 1960s. They are now used clinically for the treatment of osteoporosis, Paget's disease, bone metastasis, multiple myeloma, and other conditions that feature bone fragility.

Did you know?

The largest baby ever born weighed more than 23 pounds but died just 11 hours after his birth in 1879. The largest surviving baby was born in October 2009 in Sumatra, Indonesia, and weighed an astounding 19.2 pounds at birth.

Did you know?

Medication errors are more common among seriously ill patients than with those with minor conditions.

Did you know?

Eat fiber! A diet high in fiber can help lower cholesterol levels by as much as 10%.

Proteolytic fragments of an IgG molecule Proteolytic fragments of an IgG molecule
Forest fragments left by clear-cutting forest from the surrounding landscape have very different phy Forest fragments left by clear-cutting forest from the surrounding landscape have very different phy
Zinc-finger nucleases and TALENs bind and cut DNA at specific sequences Zinc-finger nucleases and TALENs bind and cut DNA at specific sequences
An agarose gel containing separated DNA fragments stained with the DNA-binding dye An agarose gel containing separated DNA fragments stained with the DNA-binding dye
Predicted polypeptide sequences translated from potential ORFs in the human TUBA3C gene Predicted polypeptide sequences translated from potential ORFs in the human TUBA3C gene
Phylogenetic tree constructed by comparing homologies in cytochrome c amino acid sequences Phylogenetic tree constructed by comparing homologies in cytochrome c amino acid sequences
Video: Fetal Development of the Cardiovascular System

For a complete list of videos, visit our video library