This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Assume that you have cut DNA with the restriction enzyme HindIII. You separate the fragments on an ... (Read 124 times)

jho37

  • Hero Member
  • *****
  • Posts: 531
on: Mar 26, 2020

Question 1

Nucleic acid blotting is commonly used in molecular biology. Two types, Southern blots and Northern blots, involve gel electrophoresis and a filter, which holds the nucleic acid. Briefly describe the procedure of "blotting" in this context and differentiate between Southern and Northern blots.

Question 2

Assume that you have cut λ DNA with the restriction enzyme HindIII. You separate the fragments on an agarose gel and stain the DNA with ethidium bromide. You notice that the intensity of the stain is less in the bands that have migrated closer to the "+" pole. Give an explanation for this finding.
Essentials of Genetics ¦ Klug ¦ 9th Edition
Buy Test-Bank


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by jho37 on Mar 26, 2020

TheNamesImani

  • Sr. Member
  • ****
  • Posts: 334
Reply #1 on: Mar 26, 2020
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
Answer Preview
Only 25% of students answer this correctly


jho37

  • Member
  • Posts: 531
Reply 2 on: Mar 26, 2020
Thanks for the timely response, appreciate it

lindahyatt42

  • Member
  • Posts: 322
Reply 3 on: Yesterday
Excellent

« previous next »
 

Did you know?

Normal urine is sterile. It contains fluids, salts, and waste products. It is free of bacteria, viruses, and fungi.

Did you know?

Aspirin may benefit 11 different cancers, including those of the colon, pancreas, lungs, prostate, breasts, and leukemia.

Did you know?

In 1844, Charles Goodyear obtained the first patent for a rubber condom.

Did you know?

When blood is exposed to air, it clots. Heparin allows the blood to come in direct contact with air without clotting.

Did you know?

The most common childhood diseases include croup, chickenpox, ear infections, flu, pneumonia, ringworm, respiratory syncytial virus, scabies, head lice, and asthma.

Forest fragments left by clear-cutting forest from the surrounding landscape have very different phy Forest fragments left by clear-cutting forest from the surrounding landscape have very different phy
Principles of amplification of selected fragments of DNA by polymerase chain reaction cycling. Principles of amplification of selected fragments of DNA by polymerase chain reaction cycling.
Sequencing a large chromosome by individual sequence determination of cloned fragments in sequences Sequencing a large chromosome by individual sequence determination of cloned fragments in sequences
Enzyme Enzyme
Common restriction enzymes, with their recognition sequence, DNA cutting patterns, and sources Common restriction enzymes, with their recognition sequence, DNA cutting patterns, and sources
Constructing a restriction map (2 of 5) Constructing a restriction map (2 of 5)
Video: Types of antimicrobial substances (interferons & complement protein)

For a complete list of videos, visit our video library