This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Assume that you have cut DNA with the restriction enzyme HindIII. You separate the fragments on an ... (Read 140 times)

jho37

  • Hero Member
  • *****
  • Posts: 531
on: Mar 26, 2020

Question 1

Nucleic acid blotting is commonly used in molecular biology. Two types, Southern blots and Northern blots, involve gel electrophoresis and a filter, which holds the nucleic acid. Briefly describe the procedure of "blotting" in this context and differentiate between Southern and Northern blots.

Question 2

Assume that you have cut λ DNA with the restriction enzyme HindIII. You separate the fragments on an agarose gel and stain the DNA with ethidium bromide. You notice that the intensity of the stain is less in the bands that have migrated closer to the "+" pole. Give an explanation for this finding.
Essentials of Genetics ¦ Klug ¦ 9th Edition
Buy Test-Bank


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by jho37 on Mar 26, 2020

TheNamesImani

  • Sr. Member
  • ****
  • Posts: 334
Reply #1 on: Mar 26, 2020
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
Answer Preview
Only 25% of students answer this correctly


jho37

  • Member
  • Posts: 531
Reply 2 on: Mar 26, 2020
Thanks for the timely response, appreciate it

at

  • Member
  • Posts: 359
Reply 3 on: Yesterday
Excellent

« previous next »
 

Did you know?

In 2010, opiate painkllers, such as morphine, OxyContin®, and Vicodin®, were tied to almost 60% of drug overdose deaths.

Did you know?

Critical care patients are twice as likely to receive the wrong medication. Of these errors, 20% are life-threatening, and 42% require additional life-sustaining treatments.

Did you know?

Illness; diuretics; laxative abuse; hot weather; exercise; sweating; caffeine; alcoholic beverages; starvation diets; inadequate carbohydrate consumption; and diets high in protein, salt, or fiber can cause people to become dehydrated.

Did you know?

Though newer “smart” infusion pumps are increasingly becoming more sophisticated, they cannot prevent all programming and administration errors. Health care professionals that use smart infusion pumps must still practice the rights of medication administration and have other professionals double-check all high-risk infusions.

Did you know?

Alzheimer's disease affects only about 10% of people older than 65 years of age. Most forms of decreased mental function and dementia are caused by disuse (letting the mind get lazy).

Two important changes occur in the upper airway in croup:The epiglottis swells, thereby occluding th Two important changes occur in the upper airway in croup:The epiglottis swells, thereby occluding th
Benign prostatic hyperplasia. The condition results when an inner capsule of nonfunctional prostate Benign prostatic hyperplasia. The condition results when an inner capsule of nonfunctional prostate
Heavy fuel droplets separate as they flow around an abrupt bend in an intake manifold. Heavy fuel droplets separate as they flow around an abrupt bend in an intake manifold.
Enthalpic stabilization of the transition state in an enzyme-catalyzed reaction Enthalpic stabilization of the transition state in an enzyme-catalyzed reaction
DNA fragments DNA fragments
Electrophoretic separation of a mixture of DNA fragments that vary in length Electrophoretic separation of a mixture of DNA fragments that vary in length
Video: Summation - defined, spatial, temporal & AP generation or not

For a complete list of videos, visit our video library