This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Assume that you have cut DNA with the restriction enzyme HindIII. You separate the fragments on an ... (Read 122 times)

jho37

  • Hero Member
  • *****
  • Posts: 531
on: Mar 26, 2020

Question 1

Nucleic acid blotting is commonly used in molecular biology. Two types, Southern blots and Northern blots, involve gel electrophoresis and a filter, which holds the nucleic acid. Briefly describe the procedure of "blotting" in this context and differentiate between Southern and Northern blots.

Question 2

Assume that you have cut λ DNA with the restriction enzyme HindIII. You separate the fragments on an agarose gel and stain the DNA with ethidium bromide. You notice that the intensity of the stain is less in the bands that have migrated closer to the "+" pole. Give an explanation for this finding.
Essentials of Genetics ¦ Klug ¦ 9th Edition
Buy Test-Bank


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by jho37 on Mar 26, 2020

TheNamesImani

  • Sr. Member
  • ****
  • Posts: 334
Reply #1 on: Mar 26, 2020
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
Answer Preview
Only 25% of students answer this correctly


jho37

  • Member
  • Posts: 531
Reply 2 on: Mar 26, 2020
:D TYSM

ktidd

  • Member
  • Posts: 319
Reply 3 on: Yesterday
Great answer, keep it coming :)

« previous next »
 

Did you know?

The first successful kidney transplant was performed in 1954 and occurred in Boston. A kidney from an identical twin was transplanted into his dying brother's body and was not rejected because it did not appear foreign to his body.

Did you know?

Signs of depression include feeling sad most of the time for 2 weeks or longer; loss of interest in things normally enjoyed; lack of energy; sleep and appetite disturbances; weight changes; feelings of hopelessness, helplessness, or worthlessness; an inability to make decisions; and thoughts of death and suicide.

Did you know?

The human body's pharmacokinetics are quite varied. Our hair holds onto drugs longer than our urine, blood, or saliva. For example, alcohol can be detected in the hair for up to 90 days after it was consumed. The same is true for marijuana, cocaine, ecstasy, heroin, methamphetamine, and nicotine.

Did you know?

Only one in 10 cancer deaths is caused by the primary tumor. The vast majority of cancer mortality is caused by cells breaking away from the main tumor and metastasizing to other parts of the body, such as the brain, bones, or liver.

Did you know?

Multiple experimental evidences have confirmed that at the molecular level, cancer is caused by lesions in cellular DNA.

Release of ordered water favors formation of an enzyme-substrate complex Release of ordered water favors formation of an enzyme-substrate complex
Cleaving proteins and sequencing and ordering the peptide fragments Cleaving proteins and sequencing and ordering the peptide fragments
Enzyme temperatures Enzyme temperatures
Enzyme polymorphism Enzyme polymorphism
Common restriction enzymes, with their recognition sequence, DNA cutting patterns, and sources Common restriction enzymes, with their recognition sequence, DNA cutting patterns, and sources
Enzyme activity varies with temperature Enzyme activity varies with temperature
Video: Rh blood type and complications during pregnancy & Fertilization

For a complete list of videos, visit our video library