This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Give the advantages of PCR over Southern blot and advantages of Southern blot over PCR. What will ... (Read 97 times)

D2AR0N

  • Hero Member
  • *****
  • Posts: 561
on: Jun 25, 2018
Give the advantages of PCR over Southern blot and advantages of Southern blot over PCR.
 
  What will be an ideal response?

Question 2

Explain the impact of a positive test for HTLV-1 in a lymphoma patient.
 
  What will be an ideal response?


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by a Subject Expert

kmb352

  • Sr. Member
  • ****
  • Posts: 319
Reply #1 on: Jun 25, 2018
Answer to Question 1

Answer: Advantages of PCR over Southern blot: smaller specimen volume, more rapid laboratory procedure (1-2 days vs. more than 4 days), lower cost, amenable for use on formalin-fixed paraffin-embedded tissue.
Advantages of Southern blot over PCR: no meticulous attention required to prevent carryover or contamination of laboratory samples by extraneous DNA as in the PCR procedure

Answer to Question 2

Answer: HTLV1 infects T-helper lymphocytes and causes them to proliferate by up-regulating IL-2 and its receptor. Asymptomatic HTLV1 infection is found in 15 of people in Japan and the Caribbean islands. About 0.1 of infected individuals eventually develop adult T-cell leukemia/lymphoma.


D2AR0N

  • Member
  • Posts: 561
Reply 2 on: Jun 25, 2018
:D TYSM

carlsona147

  • Member
  • Posts: 341
Reply 3 on: Yesterday
Excellent

« previous next »
 

Did you know?

For high blood pressure (hypertension), a new class of drug, called a vasopeptidase blocker (inhibitor), has been developed. It decreases blood pressure by simultaneously dilating the peripheral arteries and increasing the body's loss of salt.

Did you know?

Throughout history, plants containing cardiac steroids have been used as heart drugs and as poisons (e.g., in arrows used in combat), emetics, and diuretics.

Did you know?

In most cases, kidneys can recover from almost complete loss of function, such as in acute kidney (renal) failure.

Did you know?

The Romans did not use numerals to indicate fractions but instead used words to indicate parts of a whole.

Did you know?

It is difficult to obtain enough calcium without consuming milk or other dairy foods.

The Green Pygmy Goose (Nettapus pulchellus) is a small perching duck which breeds in southern New Gu The Green Pygmy Goose (Nettapus pulchellus) is a small perching duck which breeds in southern New Gu
Injecting an IV push (bolus) medication; C, another type of needleless syringe and needleless IV acc Injecting an IV push (bolus) medication; C, another type of needleless syringe and needleless IV acc
Southern right whale dolphins Southern right whale dolphins
Eels - That Look You Give That Guy 1 (Official Video) Eels - That Look You Give That Guy 1 (Official Video)
Video: Genetics of Cancer

For a complete list of videos, visit our video library