This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Give the advantages of PCR over Southern blot and advantages of Southern blot over PCR. What will ... (Read 159 times)

D2AR0N

  • Hero Member
  • *****
  • Posts: 561
on: Jun 25, 2018
Give the advantages of PCR over Southern blot and advantages of Southern blot over PCR.
 
  What will be an ideal response?

Question 2

Explain the impact of a positive test for HTLV-1 in a lymphoma patient.
 
  What will be an ideal response?


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by a Subject Expert

kmb352

  • Sr. Member
  • ****
  • Posts: 319
Reply #1 on: Jun 25, 2018
Answer to Question 1

Answer: Advantages of PCR over Southern blot: smaller specimen volume, more rapid laboratory procedure (1-2 days vs. more than 4 days), lower cost, amenable for use on formalin-fixed paraffin-embedded tissue.
Advantages of Southern blot over PCR: no meticulous attention required to prevent carryover or contamination of laboratory samples by extraneous DNA as in the PCR procedure

Answer to Question 2

Answer: HTLV1 infects T-helper lymphocytes and causes them to proliferate by up-regulating IL-2 and its receptor. Asymptomatic HTLV1 infection is found in 15 of people in Japan and the Caribbean islands. About 0.1 of infected individuals eventually develop adult T-cell leukemia/lymphoma.


D2AR0N

  • Member
  • Posts: 561
Reply 2 on: Jun 25, 2018
Thanks for the timely response, appreciate it

Jsherida

  • Member
  • Posts: 314
Reply 3 on: Yesterday
Great answer, keep it coming :)

« previous next »
 

Did you know?

The oldest recorded age was 122. Madame Jeanne Calment was born in France in 1875 and died in 1997. She was a vegetarian and loved olive oil, port wine, and chocolate.

Did you know?

Sildenafil (Viagra®) has two actions that may be of consequence in patients with heart disease. It can lower the blood pressure, and it can interact with nitrates. It should never be used in patients who are taking nitrates.

Did you know?

Medications that are definitely not safe to take when breastfeeding include radioactive drugs, antimetabolites, some cancer (chemotherapy) agents, bromocriptine, ergotamine, methotrexate, and cyclosporine.

Did you know?

More than 30% of American adults, and about 12% of children utilize health care approaches that were developed outside of conventional medicine.

Did you know?

Amphetamine poisoning can cause intravascular coagulation, circulatory collapse, rhabdomyolysis, ischemic colitis, acute psychosis, hyperthermia, respiratory distress syndrome, and pericarditis.

The technique of Southern blotting The technique of Southern blotting
Bobcat in the front yard of a residence in Southern California Bobcat in the front yard of a residence in Southern California
Star Map Southern Horizon Star Map Southern Horizon
Routes of Drug Administration: Advantages and Disadvantages Routes of Drug Administration: Advantages and Disadvantages
Advantages of Observational Method Advantages of Observational Method
Video: Neuron Type Animation

For a complete list of videos, visit our video library