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Author Question: Give the advantages of PCR over Southern blot and advantages of Southern blot over PCR. What will ... (Read 197 times)

D2AR0N

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on: Jun 25, 2018
Give the advantages of PCR over Southern blot and advantages of Southern blot over PCR.
 
  What will be an ideal response?

Question 2

Explain the impact of a positive test for HTLV-1 in a lymphoma patient.
 
  What will be an ideal response?


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kmb352

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Reply #1 on: Jun 25, 2018
Answer to Question 1

Answer: Advantages of PCR over Southern blot: smaller specimen volume, more rapid laboratory procedure (1-2 days vs. more than 4 days), lower cost, amenable for use on formalin-fixed paraffin-embedded tissue.
Advantages of Southern blot over PCR: no meticulous attention required to prevent carryover or contamination of laboratory samples by extraneous DNA as in the PCR procedure

Answer to Question 2

Answer: HTLV1 infects T-helper lymphocytes and causes them to proliferate by up-regulating IL-2 and its receptor. Asymptomatic HTLV1 infection is found in 15 of people in Japan and the Caribbean islands. About 0.1 of infected individuals eventually develop adult T-cell leukemia/lymphoma.


D2AR0N

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Reply 2 on: Jun 25, 2018
Great answer, keep it coming :)

amandalm

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Reply 3 on: Yesterday
Thanks for the timely response, appreciate it

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