This topic contains a solution. Click here to go to the answer

Author Question: Explain how a particular neurotransmitter receptor is localized in brain. What will be an ideal ... (Read 23 times)

penguins

  • Hero Member
  • *****
  • Posts: 903
Explain how a particular neurotransmitter receptor is localized in brain.
 
  What will be an ideal response?

Question 2

Contrast efferent and afferent tracing procedures.
 
  What will be an ideal response?



Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by a Subject Expert

Hikerman221

  • Sr. Member
  • ****
  • Posts: 346
Answer to Question 1

Answer: One way is to use autoradiography in which brain tissue is exposed to a solution of a radioactive receptor antagonist. In the second method, antibodies are proteins produced by white blood cells that are specific to another protein or peptide (antigen). Receptors are comprised of proteins (antigens), for which an antibody can be developed and tagged using a marker such as a fluorescent marker (or dye). A slice of brain tissue is incubated with the antibody/marker so that the antibody will attach to the antigen (receptor) with each location indicated by the presence of a fluorescent signal.

Answer to Question 2

Answer: In the efferent tracing procedure, a chemical such as PLA is injected into a brain site, taken up by dendrites and transported out to the axons. Immunocytochemistry is used to visualize the places that receive axons from the neurons of the injection site. In contrast, afferent tracing involves injecting a chemical, such as fluorogold, which is taken up by axon terminal buttons and transported backwards to the cell bodies. This procedure thus identifies the neurons that innervate a specific brain region.




penguins

  • Member
  • Posts: 903
Reply 2 on: Jun 22, 2018
Thanks for the timely response, appreciate it


at

  • Member
  • Posts: 359
Reply 3 on: Yesterday
Excellent

 

Did you know?

Asthma attacks and symptoms usually get started by specific triggers (such as viruses, allergies, gases, and air particles). You should talk to your doctor about these triggers and find ways to avoid or get rid of them.

Did you know?

Cutaneous mucormycosis is a rare fungal infection that has been fatal in at least 29% of cases, and in as many as 83% of cases, depending on the patient's health prior to infection. It has occurred often after natural disasters such as tornados, and early treatment is essential.

Did you know?

Thyroid conditions may make getting pregnant impossible.

Did you know?

The top 10 most important tips that will help you grow old gracefully include (1) quit smoking, (2) keep your weight down, (3) take supplements, (4) skip a meal each day or fast 1 day per week, (5) get a pet, (6) get medical help for chronic pain, (7) walk regularly, (8) reduce arguments, (9) put live plants in your living space, and (10) do some weight training.

Did you know?

Tobacco depletes the body of vitamins A, C, and E, which can result in any of the following: dry hair, dry skin, dry eyes, poor growth, night blindness, abscesses, insomnia, fatigue, reproductive system problems, sinusitis, pneumonia, frequent respiratory problems, skin disorders, weight loss, rickets, osteomalacia, nervousness, muscle spasms, leg cramps, extremity numbness, bone malformations, decayed teeth, difficulty in walking, irritability, restlessness, profuse sweating, increased uric acid (gout), joint damage, damaged red blood cells, destruction of nerves, infertility, miscarriage, and many types of cancer.

For a complete list of videos, visit our video library