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Author Question: Explain how and why the brain is preserved during the histology process. What will be an ideal ... (Read 20 times)

Brittanyd9008

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on: Jun 22, 2018
Explain how and why the brain is preserved during the histology process.
 
  What will be an ideal response?

Question 2

Explain how neuroscientists trace the efferent connections of a brain region.
 
  What will be an ideal response?


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uniquea123

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Reply #1 on: Jun 22, 2018
Answer to Question 1

Answer: The brain is perfused to remove blood from the tissue and is fixed in formalin to harden the tissue and to halt autolysis.

Answer to Question 2

Answer: A solution of PHA-L is injected into a brain region, where it is taken up by dendrites and transported to the axon terminals. After a few days, the brain of the animal is processed, using an immunocytochemical method to localize PHA-L molecules in the axon terminals. Because the axon terminals are the end point of an efferent connection, distant regions that contain nerve cells that contain PHA-L are connected with the site of injection.


Brittanyd9008

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Reply 2 on: Jun 22, 2018
Excellent

bigcheese9

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Reply 3 on: Yesterday
Thanks for the timely response, appreciate it

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