Author Question: Explain how and why the brain is preserved during the histology process. What will be an ideal ... (Read 53 times)

Brittanyd9008 · **on:** Jun 22, 2018

Explain how and why the brain is preserved during the histology process.

What will be an ideal response?

Question 2

Explain how neuroscientists trace the efferent connections of a brain region.

What will be an ideal response?

uniquea123 · **Reply #1 on:** Jun 22, 2018

Answer to Question 1

Answer: The brain is perfused to remove blood from the tissue and is fixed in formalin to harden the tissue and to halt autolysis.

Answer to Question 2

Answer: A solution of PHA-L is injected into a brain region, where it is taken up by dendrites and transported to the axon terminals. After a few days, the brain of the animal is processed, using an immunocytochemical method to localize PHA-L molecules in the axon terminals. Because the axon terminals are the end point of an efferent connection, distant regions that contain nerve cells that contain PHA-L are connected with the site of injection.